Analysis Note

ControlCONTROL ANTIGEN: Included free of charge with the antibody is 40 µg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 µL of sterile distilled water. For negative control, preincubate 1 µg of purified peptide with 1 µg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.

Application

Anti-Potassium Channel Kv4.2 Antibody is an antibody against Potassium Channel Kv4.2 for use in WB.

Research Sub CategoryIon Channels & Transporters

Research CategoryNeuroscience

Western blotting: 1:200 using ECL on rat brain membranes.

Immunohistochemistry on rat brain sections.

Immunoprecipitation Dilutions should be made using a carrier protein such as BSA (1-3%).

Optimal working dilutions must be determined by the end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

This Anti-Potassium Channel Kv4.2 Antibody is validated for use in WB for the detection of Potassium Channel Kv4.2.

Immunogen

Highly purified peptide corresponding to residues 454-469 of rat Kv4.2 (Accession Q63881).

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified immunoglobulin. Lyophilized from PBS, pH 7.4, containing 1% BSA, and 0.025% sodium azide. Reconstitute with 50 µL of sterile distilled water. Centrifuge antibody preparation before use (10,000 x g for 5 min).

Specificity

Recognizes Kv4.2 (Shal1, RK5, Kcnd2). The epitope specific for Kv4.2 is not present in any other known proteins.

SPECIES REACTIVITIES: Rat. Reactivity with other species has not yet been tested. It is expected to work on mouse and human because the immunogen sequence is highly homologous (15/16 residues identical).

Storage and Stability

Maintain lyophilized material at -20°C for up to 6 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Target description

70 kDa